Helix-Coil Transition of the Self-Complementary dG-dG-dA-dA-dT-dT-dC-dC Duplex
نویسندگان
چکیده
منابع مشابه
Pyrazolo[3,4-d]pyrimidine nucleic acids: adjustment of dA-dT to dG-dC base pair stability.
Oligonucleotides incorporating 8-aza-7-deazapurin-2,6-diamine (pyrazolo[3,4-d]pyrimidin-4,6-diamine) nucleoside 2a or its 7-bromo derivative 2b show enhanced duplex stability compared to those containing dA. While incorporation of 2a opposite dT increases the T(m) value only slightly, the 7-bromo compound 2b forms a very stable base pair which is as strong as the dG-dC pair. Compound 2b shows a...
متن کاملSpectroscopic studies on acetylaminofluorene-modified (dT-dG)n . (dC-dA)n suggest a left-handed conformation.
CD spectroscopy on the double-stranded strictly alternating dinucleotide polymer (dT-dG)n . (dC-dA)n partially modified by N-acetoxy-N-acetyl-2-aminofluorene suggests a left-handed conformation in concentrated NaCl solutions. Modification of the (dT-dG)n . (dC-dA)n polymer with acetylaminofluorene is required to promote formation of the left-handed helix since high salt concentrations and sever...
متن کاملCharge Transport in Poly(dG)-Poly(dC) and Poly(dA)-Poly(dT) DNA Polymers.
We investigate the charge transport in synthetic DNA polymers built up from single type of base pairs. In the context of a polaronlike model, for which an electronic tight-binding system and bond vibrations of the double helix are coupled, we present estimates for the electron-vibration coupling strengths utilizing a quantum-chemical procedure. Subsequent studies concerning the mobility of pola...
متن کامل(dT-dC)n and (dG-dA)n tracts arrest single stranded DNA replication in vitro.
Previous in vivo studies have indicated that (dT-dC)n.(dG-dA)n tracts (referred to here as (TC)n.(GA)n), which are widely dispersed in vertebrate genomes, may serve as pause or arrest signals for DNA replication and amplification. To determine whether these repeat elements act as stop signals for DNA replication in vitro, single stranded DNAs including (TC)n or (GA)n tracts of various lengths, ...
متن کاملHigh affinity binding of estrogen receptor to recombinant plasmids containing (dA-dC)n.(dG-dT)n sequences.
Estrogen receptor is a gene regulatory protein that is present in a subset of breast tumors and in normal sex accessory tissues of vertebrates. The receptor protein forms a high affinity complex with estradiol and mediates its action. The mechanism of action of estrogen receptor (ER) involves its binding to specific DNA sequences in the genome and the stimulation of the expression of estrogen-r...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: European Journal of Biochemistry
سال: 1979
ISSN: 0014-2956,1432-1033
DOI: 10.1111/j.1432-1033.1979.tb13037.x